| Field | Specification |
|---|---|
| Target | |
| Alternative names | Endogenous retrovirus group 3 member 1 Env polyprotein;ERV-3 envelope protein;ERV3 envelope protein;ERV3-1 envelope protein;Envelope polyprotein;HERV-R envelope protein;ERV-R envelope protein;HERV-R_7q21.2 provirus ancestral Env polyprotein;Surface protein;SU;Transmembrane protein;TM;ERV3-1;ERV3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ERV31. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets endogenous retrovirus group 3 member 1 Env polyprotein (gene ERV3-1) in human samples and is validated for Western blot. The predicted molecular weight is 67.9 kDa, and the supplier reports an observed band at about 68 kDa.
ERV3-1 encodes the envelope protein of an endogenous retrovirus that integrated into the primate genome long ago. Unlike syncytin-1, it has lost the ability to fuse membranes, but it is expressed in the placenta and other tissues, where it may influence cell growth and differentiation.
| Target | Endogenous retrovirus group 3 member 1 Env polyprotein (gene ERV3-1; UniProt Q14264, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 67.9 kDa |
| Observed MW | 68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human THP-1 cells, human K562 cells, human HepG2 cells, human U251 cells, human MCF-7 cells, human RT4 cells, human Jurkat cells, human SiHa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human ERV31.
Reactivity Notes
The supplier lists reactivity with human. UniProt places endogenous retrovirus group 3 member 1 Env polyprotein in the virion. Tissue expression noted by UniProt (human): Expressed at higher level in adrenal, sebaceous glands and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
