| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Erythropoietin; Epoetin; EPO |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human EPO recombinant protein (Position: A28-R193). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Erythropoietin EPO Antibody Picoband® is an antibody for EPO detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: EPO (erythropoietin); UniProt: P01588
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 29 kDa, calculated 52221 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Erythropoietin EPO Antibody catalog # A00484-2.
Biological background
Biological context: Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Expression and localization notes: cellular localization: Secreted., tissue context: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals..
Common research applications
- Western blotting (WB): Compare EPO levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of EPO in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify EPO-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: This EPO gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Tissue details: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
- Research category: Angiogenesis,Apoptosis,Atherosclerosis,Cardiovascular,G Protein Signaling,Hematopoietic Progenitors,Hypertrophy,Immunoglobulins,Immunology,Receptors,Signal Transduction,Signaling Pathway,Small G Proteins,Stem Cells,Surface Molecules
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
