| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trefoil factor 1;Protein pS2;Tff1;Bcei, Ps2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Tff1 recombinant protein (Position: Q22-F87). Mouse Tff1 shares 67.2% and 83.6% amino acid (aa) sequence identity with human and rat Tff1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes trefoil factor 1 (gene Tff1) in mouse samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 9.7 kDa, and the supplier reports an observed band at about 17 kDa.
Trefoil factor 1 (TFF1, also called pS2) is a small secreted peptide made mainly by gastric surface mucous cells. It helps stabilize the mucus layer that protects the stomach lining, and it is also well known as an estrogen-responsive gene in breast cancer cells.
| Target | Trefoil factor 1 (gene Tff1; UniProt Q08423, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 9.7 kDa |
| Observed MW | 17 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| ELISA (capture antibody) | 1–5 µg/mL (Mouse) |
Samples with a confirmed band (WB): mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse gaster tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse Tff1 fragment (Gln22–Phe87), expressed in E. coli. Sequence identity with the human and rat orthologs: 67.2% and 83.6%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes trefoil factor 1 as a secreted protein. Tissue expression noted by UniProt (mouse): Strong expression found in normal gastric mucosa. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xinrui Meng et al. (2024) Lamivudine protects mice from gastric ulcer by activating PGK1 to suppress ferroptosis. Biochemical Pharmacology. 10.1016/j.bcp.2024.116440
