| Field | Specification |
|---|---|
| Target | |
| Alternative names | Estrogen receptor beta;ER-beta;Nuclear receptor subfamily 3 group A member 2;ESR2;ESTRB, NR3A2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ESR2 recombinant protein (Position: F289-R501). Human ESR2 shares 93.4% and 93.9% amino acid (aa) sequence identity with mouse and rat ESR2, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects estrogen receptor beta (gene ESR2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 59.2 kDa, and the supplier reports an observed band at about 59 kDa.
Estrogen receptor beta (ESR2, ERβ) is a nuclear hormone receptor that binds estrogens with an affinity similar to ERα and activates genes containing estrogen response elements. It has a distinct tissue distribution, including the ovary, prostate and brain, and often opposes ERα-driven proliferation. Several splice isoforms exist with different activities.
| Target | Estrogen receptor beta (gene ESR2; UniProt Q92731, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 59.2 kDa |
| Observed MW | 59 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human PC-3 cells, human MCF-7 cells, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human ESR2 fragment (Phe289–Arg501), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.4% and 93.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places estrogen receptor beta in the nucleus. Tissue expression noted by UniProt (human): Expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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