| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein C-ets-1;p54;ETS1;EWSR2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ETS1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes protein C-ets-1 (gene ETS1) in human and mouse samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 50.4 kDa, and the supplier reports an observed band at about 50 kDa.
ETS1 is the founding member of the ETS family of transcription factors. It regulates genes involved in lymphocyte development and function, including cytokine and chemokine genes, as well as endothelial cell migration and angiogenesis. ETS1 is studied in immune regulation, vascular biology and tumor invasion.
| Target | Protein C-ets-1 (gene ETS1; UniProt P14921, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 50.4 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|---|
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
Samples with a confirmed band (WB): human Jurkat cells, human Daudi cells, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed result (IP): Jurkat cells.
Immunogen
Synthetic peptide from the N-terminal region of human ETS1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places protein C-ets-1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed within lymphoid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiejie Cui et al. (2020) All-trans retinoic acid reverses malignant biological behavior of hepatocarcinoma cells by regulating miR-200 family members. Genes & Diseases. 10.1016/j.gendis.2019.12.012
Dong Wang et al. (2019) Participation of tumor suppressors long non-coding RNA MEG3, microRNA-377 and PTEN in glioma cell invasion and migration. Pathology Research and Practice. 10.1016/j.prp.2019.152558
