Anti-EVL Antibody Picoband®

Overview

Anti-EVL Antibody Picoband® is an antibody for EVL detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: EVL (tumor necrosis factor (ligand) superfamily, member 4); UniProt: Q9UI08
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 50 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-EVL Antibody Picoband® catalog # A02568-2.

Biological background

Biological context: Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.

Expression and localization notes: cellular localization: Membrane; Single-pass type II membrane protein., tissue context: Macrophages; peripheral blood leukocytes, lung, spleen and liver..

Common research applications

• Western blotting (WB): Compare EVL levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of EVL in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify EVL-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Ena/VASP-like protein is a member of the Ena/VASP family of proteins that in humans is encoded by the EVL gene. Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells. The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization. Research studies have shown that EVL also regulates DNA repair by interaction with RAD51. EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination. Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane; Single-pass type II membrane protein.
• Tissue details: Macrophages; peripheral blood leukocytes, lung, spleen and liver.
• Research category: Adaptive Immunity,Autoimmune,B Cells,Cell Type Markers,Cytokines,Immune System Diseases,Immunology,Innate Immunity,T Cells,TNF Superfamily

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.