Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4)

Overview

This antibody is intended for detection of EWSR1 in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.

Vendor notes: Boster Bio Anti-EWSR1 Antibody Picoband® (monoclonal, 4B4) catalog # M00589. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Key elements and design rationale

• Antibody format: Mouse Monoclonal Mouse IgG2b
• Clone number: Clone: 4B4
• Immunogen / epitope context: A synthetic peptide corresponding to a sequence in the middle region of human EWSR1, different from the related mouse sequence by one amino acid.
• Molecular weight context: reported MW: 95 kDa; calculated MW: nan
• Reactivity: Human,Monkey,Mouse
• Applications: IHC, WB

As a monoclonal antibody, the reagent targets a defined epitope, supporting consistency across experiments; epitope masking by PTMs or conformational changes can affect signal.

Biological background

EWS RNA-binding protein 1. This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t (11;22) (q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. Functional note: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. Reported localization: Nucleus Expression/tissue context: Ubiquitous.

Research relevance and current trends

• Cancer Susceptibility: Researchers commonly examine how EWSR1 relates to this theme using model systems and orthogonal readouts.
• Cell Type Markers: Researchers commonly examine how EWSR1 relates to this theme using model systems and orthogonal readouts.
• Chromatin Binding Proteins: Researchers commonly examine how EWSR1 relates to this theme using model systems and orthogonal readouts.

Common research applications

• Western blotting: compare relative EWSR1 levels across conditions; band patterns may reflect isoforms and processing.
• IHC/IHC-F: assess spatial distribution of EWSR1 across tissue regions and cell types using matched controls.

Notes for experimental interpretation

• Specificity notes: No cross reactivity with other proteins.
• Cross-reactivity: No cross-reactivity with other proteins.
• Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
• Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.