| Field | Specification |
|---|---|
| Target | |
| Alternative names | Exportin-5; Exp5; Ran-binding protein 21; XPO5; KIAA1291; RANBP21 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Exportin 5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20X50 is a rabbit monoclonal antibody that recognizes exportin-5 (gene XPO5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 136.3 kDa, and the supplier reports an observed band at about 136 kDa.
Exportin-5 (XPO5) is a Ran-GTP-dependent nuclear export receptor that recognizes double-stranded RNA structures. Its best-known cargo is pre-microRNA hairpins produced by Drosha, which it carries to the cytoplasm for Dicer processing, and it also exports certain tRNAs and dsRNA-binding proteins, so it is central to studies of microRNA biogenesis.
| Target | Exportin-5 (gene XPO5; UniProt Q9HAV4, human) |
|---|---|
| Clone | 20X50 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 136.3 kDa |
| Observed MW | 136 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells, NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human exportin 5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places exportin-5 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
