| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein phosphatase EYA2; Eyes absent homolog 2; EYA2; EAB1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human EYA2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28E31 is a rabbit monoclonal antibody that recognizes protein phosphatase EYA2 in human samples and is validated for Western blot. The predicted molecular weight is 59.2 kDa, and the supplier reports an observed band at about 60 kDa.
EYA2 is a member of the Eyes absent family, proteins that combine transcriptional coactivator and protein phosphatase activities. It partners with SIX homeodomain factors to drive developmental gene programs, and it dephosphorylates histone H2AX at tyrosine 142 to favor DNA repair over apoptosis. EYA2 is studied in development and cancer.
| Target | Protein phosphatase EYA2 (gene EYA2; UniProt O00167, human) |
|---|---|
| Clone | 28E31 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.2 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human EYA2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places protein phosphatase EYA2 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
