| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fatty-acid amide hydrolase 1; Anandamide amidohydrolase 1; Fatty acid ester hydrolase; Oleamide hydrolase 1; FAAH; FAAH1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FAAH1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20F33 is a rabbit monoclonal antibody that recognizes fatty-acid amide hydrolase 1 (gene FAAH) in human and mouse samples and is validated for Western blot and IHC. The predicted molecular weight is 63.1 kDa, and the supplier reports an observed band at about 63 kDa.
Fatty acid amide hydrolase (FAAH) is a membrane-bound serine hydrolase that breaks down fatty acid amides, including the endocannabinoid anandamide and the sleep-inducing lipid oleamide. By ending the signaling of these lipids, FAAH regulates endocannabinoid tone, and it is studied in pain, anxiety and inflammation research.
| Target | Fatty-acid amide hydrolase 1 (gene FAAH; UniProt O00519, human) |
|---|---|
| Clone | 20F33 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 63.1 kDa |
| Observed MW | 63 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human SH-SY5Y cells, mouse brain tissue, mouse pancreas tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FAAH1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places fatty-acid amide hydrolase 1 in the endomembrane system and cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
