| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fatty acid-binding protein, intestinal;Fatty acid-binding protein 2;Intestinal-type fatty acid-binding protein;I-FABP;Fabp2;Fabpi; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse FABP2/I-FABP recombinant protein (Position: A2-E132). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FABP2/I-FABP Antibody is an antibody for Fabp2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Fabp2 (Fatty acid-binding protein, intestinal); UniProt: P55050
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 15 kDa, calculated 15126 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FABP2/I-FABP Antibody Picoband® catalog # A02378-1.
Biological background
Biological context: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. .
Expression and localization notes: cellular localization: Cytoplasm., tissue context: Expressed in the small intestine. Highest expression levels in the proximal ileum. ..
Common research applications
- Western blotting (WB): Compare Fabp2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Fabp2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Fabp2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: FABP 2, Fatty acid-binding protein 2, is a protein that in humans is encoded by the FABP2 gene. Using a human cDNA probe, the gene is assigned to chromosome 4 in somatic cell hybrids. FABP 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. The FABPs belong to a multigene family with nearly twenty identified members. And FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. Also, they may be responsible in the modulation of cell growth and proliferation.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Cytoplasm.
- Tissue details: Expressed in the small intestine. Highest expression levels in the proximal ileum. .
- Research category: Calcium Binding Proteins,Calcium Signaling,Neuroscience,Neurotransmission
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
