| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fatty acid-binding protein, intestinal;Fatty acid-binding protein 2;Intestinal-type fatty acid-binding protein;I-FABP;Fabp2;Fabpi; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived mouse FABP2/I-FABP recombinant protein (Position: A2-E132). Mouse FABP2/I-FABP shares 77.9% and 92.4% amino acid (aa) sequence identity with human and rat FABP2/I-FABP, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Fabp2 (Fatty acid-binding protein, intestinal) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FABP2/I-FABP Antibody Picoband® catalog # A02378. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived mouse FABP2/I-FABP recombinant protein (Position: A2-E132). Mouse FABP2/I-FABP shares 77.9% and 92.4% amino acid (aa) sequence identity with human and rat FABP2/I-FABP, respectively. (reported region: A2-E132).
- Molecular weight context: reported MW: 15 kDa; calculated MW: 15126 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Fatty acid-binding protein, intestinal; Fatty acid-binding protein, intestinal. FABP 2, Fatty acid-binding protein 2, is a protein that in humans is encoded by the FABP2 gene. Using a human cDNA probe, the gene is assigned to chromosome 4 in somatic cell hybrids. FABP 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. The FABPs belong to a multigene family with nearly twenty identified members. And FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. Also, they may be responsible in the modulation of cell growth and proliferation. Functional note: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. . Reported localization: Cytoplasm. Expression/tissue context: Expressed in the small intestine. Highest expression levels in the proximal ileum. .
Research relevance and current trends
- Calcium Binding Proteins: Researchers commonly examine how Fabp2 (Fatty acid-binding protein, intestinal) relates to this theme using model systems and orthogonal readouts.
- Calcium Signaling: Researchers commonly examine how Fabp2 (Fatty acid-binding protein, intestinal) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how Fabp2 (Fatty acid-binding protein, intestinal) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Fabp2 (Fatty acid-binding protein, intestinal) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of Fabp2 (Fatty acid-binding protein, intestinal) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
