| Field | Specification |
|---|---|
| Target | |
| Alternative names | Complement factor D;3.4.21.46;28 kDa adipocyte protein;Adipsin;C3 convertase activator;Properdin factor D;Cfd;Adn, Df; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Factor D recombinant protein (Position: Q30-K235). Mouse Factor D shares 66.5% and 87.4% amino acid (aa) sequence identity with human and rat Factor D, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes complement factor D (gene Cfd) in mouse samples and is validated for Western blot. The predicted molecular weight is 28.1 kDa, and the supplier reports an observed band at about 43 kDa.
Complement factor D (CFD, adipsin) is a serine protease that starts the alternative complement pathway by cleaving factor B bound to C3b. It is produced mainly by adipocytes and circulates in plasma, and it is studied in innate immunity, complement disorders and adipose tissue biology.
| Target | Complement factor D (gene Cfd; UniProt P03953, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 28.1 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse) |
|---|
Samples with a confirmed band (WB): mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Factor D fragment (Gln30–Lys235), expressed in E. coli. Sequence identity with the human and rat orthologs: 66.5% and 87.4%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes complement factor D as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
