| Field | Specification |
|---|---|
| Target | |
| Alternative names | Coagulation factor VII;3.4.21.21;Proconvertin;Serum prothrombin conversion accelerator;SPCA;Eptacog alfa;Factor VII light chain;Factor VII heavy chain;F7; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets coagulation factor VII (gene F7) in human samples and is validated for Western blot. The predicted molecular weight is 51.6 kDa, and the supplier reports an observed band at about 52 kDa.
Coagulation factor VII (F7) is a vitamin K-dependent serine protease that circulates mostly as an inactive zymogen. Bound to tissue factor in the presence of calcium, activated factor VIIa initiates the extrinsic pathway by activating factors IX and X. Factor VII deficiency is a rare bleeding disorder, and recombinant factor VIIa is used clinically to treat certain bleeding conditions.
| Target | Coagulation factor VII (gene F7; UniProt P08709, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 51.6 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): SMMC cells, Jurkat cells, RAJI cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide from the C-terminal region of human factor VII.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes coagulation factor VII as a secreted protein. Tissue expression noted by UniProt (human): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
