| Field | Specification |
|---|---|
| Target | |
| Alternative names | Coagulation factor X; Stuart factor; Stuart-Prower factor; F10 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Factor X |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18F04 is a rabbit monoclonal antibody that recognizes coagulation factor X (gene F10) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 54.7 kDa, and the supplier reports an observed band at about 74 kDa.
Coagulation factor X (F10) is a vitamin K-dependent serine protease precursor that sits where the intrinsic and extrinsic clotting pathways meet. Activated factor Xa, together with factor Va, calcium and phospholipid, forms the prothrombinase complex that converts prothrombin to thrombin. Factor Xa also signals through protease-activated receptors and is the target of direct oral anticoagulants.
| Target | Coagulation factor X (gene F10; UniProt P00742, human) |
|---|---|
| Clone | 18F04 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.7 kDa |
| Observed MW | 74 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human factor X.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes coagulation factor X as a secreted protein. Tissue expression noted by UniProt (human): Plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
