| Field | Specification |
|---|---|
| Target | |
| Alternative names | FAS-associated death domain protein;FAS-associating death domain-containing protein;Growth-inhibiting gene 3 protein;Mediator of receptor induced toxicity;Protein FADD;FADD;MORT1;GIG3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FADD. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets FAS-associated death domain protein (gene FADD) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 23.3 kDa, and the supplier reports an observed band at about 28 kDa.
FADD is an adaptor protein with a death domain and a death effector domain. It is recruited to activated death receptors such as FAS and TNFR1, where it brings in caspase-8 or caspase-10 to form the death-inducing signaling complex (DISC). FADD is essential for extrinsic apoptosis and also regulates necroptosis and immune signaling.
| Target | FAS-associated death domain protein (gene FADD; UniProt Q13158, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 23.3 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HT1080 cells, human A549 cells, human Jurkat cells, human A431 cells, human HepG2 cells, human HeLa cells, human MCF-7 cells, human Raji cells, rat spleen tissue, rat PC-12 cells, mouse spleen tissue, mouse kidney tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human FADD.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places FAS-associated death domain protein in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yan-Qing Guan et al. (2010) Death signal transduction induced by co-immobilized TNF-α plus IFN-γ and the development of polymeric anti-cancer drugs. Biomaterials. 10.1016/j.biomaterials.2010.08.044
Lin Shi et al. (2018) Scutellarein selectively targets multiple myeloma cells by increasing mitochondrial superoxide production and activating intrinsic apoptosis pathway. Biomedicine & Pharmacotherapy. 10.1016/j.biopha.2018.09.024
Qihui Luo et al. (2018) Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. Biomed Research International. 10.1155/2018/5241941
Hui Wang et al. (2022) Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line. Research in Veterinary Science. 10.1016/j.rvsc.2022.01.001
Liang Zhang et al. (2007) A cytotoxin isolated from Agkistrodon acutus snake venom induces apoptosis via Fas pathway in A549 cells. Toxicology in Vitro. 10.1016/j.tiv.2007.04.008
