Anti-FAM129B/NIBAN2 Antibody Picoband®

Overview

Anti-FAM129B/NIBAN2 Antibody Picoband® is an antibody for NIBAN2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: NIBAN2 (CD72 molecule); UniProt: Q96TA1
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 95 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-FAM129B/NIBAN2 Antibody Picoband® catalog # A09391-2.

Biological background

Biological context: Plays a role in B-cell proliferation and differentiation.

Expression and localization notes: cellular localization: Membrane; Single-pass type II membrane protein., tissue context: Pre-B-cells and B-cells but not terminally differentiated plasma cells..

Common research applications

• Western blotting (WB): Compare NIBAN2 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of NIBAN2 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify NIBAN2-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: FAM129B/Niban-like protein 1 (family with sequence similarity 129, member B) belongs to a poorly characterized family of Niban proteins that also includes FAM129A/Niban and FAM129C/Niban-like protein 2. FAM129A/Niban is implicated in the ER stress response and is upregulated at the protein level in thyroid carcinoma. FAM129C/Niban-like protein 2 is preferentially expressed in B-cells and is one of five biomarkers upregulated in whole blood from patients with colorectal carcinoma. FAM129B is broadly expressed and has been shown to be a downstream target of B-Raf in melanoma cells. Though FAM129B does not appear to regulate cell growth and division, phosphorylation of FAM129B by B-Raf is essential for the invasive potential of melanoma and non-melanoma cell lines. Deletion of FAM129B in melanoma cells significantly impairs B-Raf/MEK/Erk-dependent invasion into the extracellular matrix.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Membrane; Single-pass type II membrane protein.
• Tissue details: Pre-B-cells and B-cells but not terminally differentiated plasma cells.
• Research category: Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunology,Lymphoid,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.