| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;FAS;APT1, FAS1, TNFRSF6; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Fas recombinant protein (Position: Q26-N173). Human Fas shares 55% and 59% amino acid (aa) sequences identity with mouse and rat Fas, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes tumor necrosis factor receptor superfamily member 6 (gene FAS) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 37.7 kDa, and the supplier reports an observed band at about 37 kDa.
Fas (CD95, TNFRSF6) is a death receptor of the TNF receptor superfamily. When bound by Fas ligand, it recruits the adaptor FADD and caspase-8 to form a signaling complex that starts the caspase cascade and apoptosis. Fas is studied in immune homeostasis, activation-induced T-cell death, autoimmunity and tumor biology.
| Target | Tumor necrosis factor receptor superfamily member 6 (gene FAS; UniProt P25445, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 37.7 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells, Jurkat cells, A549 cells, SMMC cells, K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Recombinant human Fas fragment (Gln26–Asn173), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 55% and 59%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lv Yanhong et al. (2017) Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma. Scientific Reports. 10.1038/srep40967
Dimas Angelos et al. (2023) The expression of corticotropin-releasing hormone family peptides in premalignant and malignant vulvar lesions. Clinical & Translational Oncology. 10.1007/s12094-023-03249-8
Yaojie Zheng et al. (2016) The effect of geniste on Aβ25–35-induced PC12 cell apoptosis through the JNK-dependent Fas pathway. Food & Function. 10.1039/C6FO00071A
Shuhang Liang et al. (2016) Role of Cyt-C/caspases-9,3, Bax/Bcl-2 and the FAS death receptor pathway in apoptosis induced by zinc oxide nanoparticles in human aortic endothelial cells and the protective effect by alpha-lipoic acid. Chemico-Biological Interactions. 10.1016/j.cbi.2016.08.013
Qihui Luo et al. (2018) Role of the Death Receptor and Endoplasmic Reticulum Stress Signaling Pathways in Polyphyllin I-Regulated Apoptosis of Human Hepatocellular Carcinoma HepG2 Cells. Biomed Research International. 10.1155/2018/5241941
Qian Li et al. (2010) Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues. World Journal of Gastroenterology. 10.3748/wjg.v16.i10.1274
Yin Li et al. (2015) Significance of Fas and FasL protein expression in cardiac carcinoma and local lymph node tissues. International Journal of Clinical and Experimental Pathology. PMID: 26617948
Li Wei et al. (2011) Relationship between aberrant methylation of FAS promoter and biological behavior of bladder urothelial carcinoma. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-011-0679-6
