| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen: CD95; Fas; Apt1; Tnfrsf6 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E.coli-derived mouse Fas recombinant protein (Position: E46-K279). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Fas Antibody Picoband® is an antibody for Fas detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: Fas (Fas (TNF receptor superfamily member 6)); UniProt: P25446; NCBI Gene: 355
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 37 kDa, calculated 25283 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Fas Antibody Picoband® catalog # A00055.
Biological background
Biological context: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
Expression and localization notes: cellular localization: Cell membrane. Single-pass type I membrane protein., tissue context: Detected in various tissues including thymus, liver, lung, heart, and adult ovary..
Common research applications
- Western blotting (WB): Compare Fas levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of Fas in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify Fas-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: FAS (also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. FAS antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor (TNF) receptors and the low-affinity nerve growth factor receptor (NGFR) and it is mapped to 10q24.1. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Cell membrane. Single-pass type I membrane protein.
- Tissue details: Detected in various tissues including thymus, liver, lung, heart, and adult ovary.
- Research category: Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
