| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD_antigen: CD95; Fas; Apt1; Tnfrsf6 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Fas recombinant protein (Position: E46-K279). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tumor necrosis factor receptor superfamily member 6 (gene Fas) in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 37.4 kDa, and the supplier reports an observed band at about 37 kDa.
Fas (CD95, TNFRSF6) is a death receptor of the TNF receptor superfamily. When bound by Fas ligand, it recruits the adaptor FADD and caspase-8 to form a signaling complex that starts the caspase cascade and apoptosis. Fas is studied in immune homeostasis, activation-induced T-cell death, autoimmunity and tumor biology.
| Target | Tumor necrosis factor receptor superfamily member 6 (gene Fas; UniProt P25446, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 37.4 kDa |
| Observed MW | 37 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat thymus tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): RAW264.7 cells.
Immunogen
Recombinant mouse Fas fragment (Glu46–Lys279), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places tumor necrosis factor receptor superfamily member 6 in the cell membrane and membrane raft. Tissue expression noted by UniProt (mouse): Detected in various tissues including thymus, liver, lung, heart, and adult ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Jun Quan Li et al. (2024) Understanding the relationship between inflammation, apoptosis, and diabetes osteoporosis: A bioinformatics approach and experimental verification. FASEB Journal. 10.1096/fj.202401452R
Zhang Yan et al. (2021) Perfluorooctanoic acid exposure in early pregnancy induces oxidative stress in mice uterus and liver. Environmental Science and Pollution Research. 10.1007/s11356-021-15453-6
Wang Xiaohui et al. (2026) Decreasing S1PR3 Alleviated Renal Tubular Lipid Deposition in Diabetic Nephropathy via AMPK/PPAR-γ/GPAT3 Pathway. Applied Biochemistry and Biotechnology. 10.1007/s12010-026-05640-x
Kun Gao et al. (2008) Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation. Cell Biochemistry and Function. 10.1002/cbf.1523
