| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;Fas;Apt1, Tnfrsf6; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets tumor necrosis factor receptor superfamily member 6 (gene Fas) in mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 36.8 kDa, and the supplier reports an observed band at about 12 kDa.
Fas (CD95, TNFRSF6) is a death receptor of the TNF receptor superfamily. When bound by Fas ligand, it recruits the adaptor FADD and caspase-8 to form a signaling complex that starts the caspase cascade and apoptosis. Fas is studied in immune homeostasis, activation-induced T-cell death, autoimmunity and tumor biology.
| Target | Tumor necrosis factor receptor superfamily member 6 (gene Fas; UniProt Q63199, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 36.8 kDa |
| Observed MW | 12 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat liver extract, rat spleen extract, rat brain extract, rat cardiac muscle extract, mouse liver extract, mouse spleen extract, mouse brain extract, mouse kidney extract, mouse thymus extract, mouse lung extract, HEPA1–6 cells, NIH3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): rat spleen tissue, mouse intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the N-terminal region of rat fas.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places tumor necrosis factor receptor superfamily member 6 in the cell membrane and membrane raft. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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T. Weng et al. (2014) Loss of Vhl in cartilage accelerated the progression of age-associated and surgically induced murine osteoarthritis. Osteoarthritis and Cartilage. 10.1016/j.joca.2014.06.031
M. H. Soomro et al. (2017) Molecular and structural changes related to hepatitis E virus antigen and its expression in testis inducing apoptosis in Mongolian gerbil model. Journal of Viral Hepatitis. 10.1111/jvh.12690
Gao Xiujie et al. (2015) HSP70 inhibits stress-induced cardiomyocyte apoptosis by competitively binding to FAF1. Cell Stress & Chaperones. 10.1007/s12192-015-0589-9
Hui Wang et al. (2022) Aconitine induces cell apoptosis via mitochondria and death receptor signaling pathways in hippocampus cell line. Research in Veterinary Science. 10.1016/j.rvsc.2022.01.001
Wei Liu et al. (2013) Combined early fluid resuscitation and hydrogen inhalation attenuates lung and intestine injury. World Journal of Gastroenterology. 10.3748/wjg.v19.i4.492
