| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fascin-2; Retinal fascin; FSCN2 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Fascin 2/FSCN2 recombinant protein (Position: M1-K167). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Fascin 2/FSCN2 Antibody Picoband® is an antibody for FSCN2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FSCN2 (fascin actin-bundling protein 2, retinal); UniProt: O14926
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 60 kDa, calculated 49229 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-Fascin 2/FSCN2 Antibody Picoband® catalog # A07840-2.
Biological background
Biological context: Acts as an actin bundling protein. May play a pivotal role in photoreceptor cell-specific events, such as disk morphogenesis.
Expression and localization notes: cellular localization: Nucleus., tissue context: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina..
Common research applications
- Western blotting (WB): Compare FSCN2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FSCN2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify FSCN2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Specificity: No cross reactivity with other proteins.
- Background: Fascin-2 is a protein that in humans is encoded by the FSCN2 gene. It is mapped to 17q25.3. This gene encodes a member of the fascin protein family. Fascins crosslink actin into filamentous bundles within dynamic cell extensions. This family member is proposed to play a role in photoreceptor disk morphogenesis. A mutation in this gene results in one form of autosomal dominant retinitis pigmentosa and macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus.
- Tissue details: Localized specifically in the outer and inner segments of the photoreceptor cells in the retina.
- Research category: Golgi Proteins,Protein Trafficking,Signal Transduction,Vesicle Transport
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
