| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fatty acid synthase;2.3.1.85;[Acyl-carrier-protein] S-acetyltransferase;2.3.1.38;[Acyl-carrier-protein] S-malonyltransferase;2.3.1.39;3-oxoacyl-[acyl-carrier-protein] synthase;2.3.1.41;3-oxoacyl-[acyl-carrier-protein] reductase;1.1.1.100;3-hydroxyacyl-[acyl-carrier-protein] dehydratase;4.2.1.59;Enoyl-[acyl-carrier-protein] reductase;1.3.1.39;Oleoyl-[acyl-carrier-protein] hydrolase;3.1.2.14;FASN;FAS; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FASN (Fatty acid synthase) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Fatty Acid Synthase/FASN Antibody Picoband® catalog # PB9865. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively. (reported region: M1-E226).
- Molecular weight context: reported MW: 273 kDa; calculated MW: 273427 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Fatty acid synthase; Fatty acid synthase. Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. Functional note: Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein. Reported localization: Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Expression/tissue context: Ubiquitous. Prominent expression in brain, lung, and liver. .
Research relevance and current trends
- Cancer: Researchers commonly examine how FASN (Fatty acid synthase) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how FASN (Fatty acid synthase) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how FASN (Fatty acid synthase) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FASN (Fatty acid synthase) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of FASN (Fatty acid synthase) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
