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| UniProt # |
Immunotherapy
Product Overview
Anti-FcGR3A-anti-EGFR bispecific IgG antibody, which recognizes FcGR3A (also known as CD16A) and EGFR antigen binding proteins. The amino acid sequences correspond to the sequences from the bispecific EGFR/FcGR3 antigen-binding protein patent WO2019175368A1. This bispecific antibody has been tested for specific binding affinity to purified human FcGR3A and EGFR recombinant protein.
| Clonality | Monoclonal (Bispecific) |
|---|---|
| Host | Human |
| Antibody Type | IgG |
| Reactivity | Human |
| Purity | ≥90% |
| Molecular Weight | Heavy Chain: 76 kDa; Light Chain: 23 kDa + glycans |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol |
| UniProt | FcGR3A: P08637; EGFR: P00533 |
| Storage | At least 12 months at -80°C. |
Validated Applications
- ELISA
Assay conditions: Functional validation: The antibody was validated by measuring anti-FcGR3A-anti-EGFR binding to FcGR3A and EGFR antigens in ELISA assay. The FcGR3A protein (BPS Bioscience #79013) and EGFR protein were coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-FcGR3A-anti-EGFR antibody (BPS Bioscience #101535), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Usage note: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Avoid freeze/thaw cycles.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 100173
- 100689
- 100761
- 101219
This antibody has been validated for: ELISA. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at At least 12 months at -80°C.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
