| Field | Specification |
|---|---|
| Target | |
| Alternative names | IgG receptor FcRn large subunit p51;FcRn;IgG Fc fragment receptor transporter alpha chain;Neonatal Fc receptor;Fcgrt;Fcrn; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse FCGRT recombinant protein (Position: Q54-D291). Mouse FCGRT shares 70.2% and 92.4% amino acid (aa) sequence identity with human and rat FCGRT, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets igG receptor FcRn large subunit p51 (gene Fcgrt) in mouse samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 40.1 kDa, and the supplier reports an observed band at about 50 kDa.
The neonatal Fc receptor (FcRn, FCGRT) is an MHC class I-like receptor that pairs with β2-microglobulin and binds IgG and albumin in a pH-dependent manner. It transfers maternal IgG to the fetus or newborn and protects IgG and albumin from degradation, which extends their half-life in circulation. FcRn is widely studied in antibody pharmacokinetics.
| Target | IgG receptor FcRn large subunit p51 (gene Fcgrt; UniProt Q61559, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 40.1 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
| Western blot | 0.1–0.5 µg/mL (Mouse) |
Samples with a confirmed band (WB): mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse FCGRT fragment (Gln54–Asp291), expressed in E. coli. Sequence identity with the human and rat orthologs: 70.2% and 92.4%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt places igG receptor FcRn large subunit p51 in the cell membrane and endosome membrane. Tissue expression noted by UniProt (mouse): Intestinal epithelium of suckling rodents. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
