| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human FGF19 recombinant protein (Position: L25-K216). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets fibroblast growth factor 19 (gene FGF19) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 24.0 kDa, and the supplier reports an observed band at about 24 kDa.
Fibroblast growth factor 19 (FGF19; the mouse ortholog is Fgf15) is an endocrine FGF released from the small intestine after meals in response to bile acids. Acting on liver FGFR4 together with the coreceptor β-Klotho, it suppresses bile acid synthesis by lowering CYP7A1 expression and also influences glucose and lipid metabolism. It is studied in bile acid homeostasis and metabolic disease.
| Target | Fibroblast growth factor 19 (gene FGF19; UniProt O95750, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 24.0 kDa |
| Observed MW | 24 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human FGF19 fragment (Leu25–Lys216), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes fibroblast growth factor 19 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in fetal brain, cartilage, retina, and adult gall bladder. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
