| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat Fgf19(Fgf15) recombinant protein (Position: R26-K218). Rat Fgf19(Fgf15) shares 53.2% and 96.4% amino acid (aa) sequence identity with human and mouse FGF19, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects fibroblast growth factor (gene Fgf19) in mouse and rat samples and is validated for IHC.
Fibroblast growth factor 19 (FGF19; the mouse ortholog is Fgf15) is an endocrine FGF released from the small intestine after meals in response to bile acids. Acting on liver FGFR4 together with the coreceptor β-Klotho, it suppresses bile acid synthesis by lowering CYP7A1 expression and also influences glucose and lipid metabolism. It is studied in bile acid homeostasis and metabolic disease.
| Target | Fibroblast growth factor (gene Fgf19; UniProt Q8VI81, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
|---|
Samples with confirmed staining (IHC): mouse colon tissue, rat colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant rat Fgf19(Fgf15) fragment (Arg26–Lys218), expressed in E. coli. Sequence identity with the human and mouse orthologs: 53.2% and 96.4%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes fibroblast growth factor as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lu Xu et al. (2023) Zhuyu Pill Alleviates Nonalcoholic Fatty Liver Disease by Regulating Bile Acid Metabolism through the Gut–Liver Axis. ACS Omega. 10.1021/acsomega.3c01955