Anti-Fgf19(Fgf15) Antibody

SKU:BHA21000967
Research Validated
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Overview
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Anti-Fgf19(Fgf15) antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Mouse, Rat. Recognizes fibroblast growth factor. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Fgf19(Fgf15)
Host Rabbit
Reactivity Mouse,Rat
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
PB10062 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IHC
      Application details: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
      Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Fgf19(Fgf15)
Alternative names Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533;
Gene ID 170582/14170
UniProt # Q8VI81
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
Immunogen E. coli-derived rat Fgf19(Fgf15) recombinant protein (Position: R26-K218). Rat Fgf19(Fgf15) shares 53.2% and 96.4% amino acid (aa) sequence identity with human and mouse FGF19, respectively.
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Secreted.
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) PB10062
Main SKU BHA21000967
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody detects fibroblast growth factor (gene Fgf19) in mouse and rat samples and is validated for IHC.

Fibroblast growth factor 19 (FGF19; the mouse ortholog is Fgf15) is an endocrine FGF released from the small intestine after meals in response to bile acids. Acting on liver FGFR4 together with the coreceptor β-Klotho, it suppresses bile acid synthesis by lowering CYP7A1 expression and also influences glucose and lipid metabolism. It is studied in bile acid homeostasis and metabolic disease.

Target Fibroblast growth factor (gene Fgf19; UniProt Q8VI81, rat)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Mouse, Rat
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (paraffin sections) 2–5 µg/mL (Mouse, Rat)

Samples with confirmed staining (IHC): mouse colon tissue, rat colon tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).

Immunogen

Recombinant rat Fgf19(Fgf15) fragment (Arg26–Lys218), expressed in E. coli. Sequence identity with the human and mouse orthologs: 53.2% and 96.4%, respectively.

Reactivity Notes

The supplier lists reactivity with mouse and rat. UniProt describes fibroblast growth factor as a secreted protein.

Safety

For research use only; not for diagnostic or therapeutic procedures. Handle with standard laboratory precautions.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 2–5 µg/mL (Mouse, Rat). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Mouse, Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant rat Fgf19(Fgf15) fragment (Arg26–Lys218), expressed in E. coli. Sequence identity with the human and mouse orthologs: 53.2% and 96.4%, respectively.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Lu Xu et al. (2023) Zhuyu Pill Alleviates Nonalcoholic Fatty Liver Disease by Regulating Bile Acid Metabolism through the Gut–Liver Axis. ACS Omega. 10.1021/acsomega.3c01955

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