| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | E. coli-derived rat Fgf19(Fgf15) recombinant protein (Position: R26-K218). Rat Fgf19(Fgf15) shares 53.2% and 96.4% amino acid (aa) sequence identity with human and mouse FGF19, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Fgf19(Fgf15) (Fibroblast growth factor 19) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Fgf19(Fgf15) Antibody Picoband® catalog # PB10062. Tested in IHC applications. This antibody reacts with Mouse, Rat.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived rat Fgf19(Fgf15) recombinant protein (Position: R26-K218). Rat Fgf19(Fgf15) shares 53.2% and 96.4% amino acid (aa) sequence identity with human and mouse FGF19, respectively. (reported region: R26-K218).
- Molecular weight context: reported MW: 24 kDa; calculated MW: 24003 MW
- Reactivity: Mouse,Rat
- Applications: IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Fibroblast growth factor 19; Fibroblast growth factor 19. FGF15/19 refers to two orthologous fibroblast growth factors which share 50% aminoacid identity and have similar functions. FGF15 was described in the mouse; FGF19 was found in humans and other species. They share physiological functions and so are often referred to as FGF15/19 or as FGF15/FGF19. They were first described in developing fetal brain. They are now known to be produced in the ileum, and under certain circumstances in the liver and biliary tree. It is thought their principal function is in response to bile acid absorption occurring after meals. FGF15 and FGF19 have similar roles in regulating bile acid synthesis and also glucose metabolism in the liver. Functional note: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. . Reported localization: Secreted. Expression/tissue context: Expressed in fetal brain, cartilage, retina, and adult gall bladder. .
Research relevance and current trends
- Cancer: Researchers commonly examine how Fgf19(Fgf15) (Fibroblast growth factor 19) relates to this theme using model systems and orthogonal readouts.
- Growth Factors: Researchers commonly examine how Fgf19(Fgf15) (Fibroblast growth factor 19) relates to this theme using model systems and orthogonal readouts.
- Growth Factors/Hormones: Researchers commonly examine how Fgf19(Fgf15) (Fibroblast growth factor 19) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of Fgf19(Fgf15) (Fibroblast growth factor 19) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
