| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor 19; FGF-19; FGF19 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FGF19 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24F22 is a rabbit monoclonal antibody that recognizes fibroblast growth factor 19 (gene FGF19) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 24.0 kDa, and the supplier reports an observed band at about 24 kDa.
Fibroblast growth factor 19 (FGF19; the mouse ortholog is Fgf15) is an endocrine FGF released from the small intestine after meals in response to bile acids. Acting on liver FGFR4 together with the coreceptor β-Klotho, it suppresses bile acid synthesis by lowering CYP7A1 expression and also influences glucose and lipid metabolism. It is studied in bile acid homeostasis and metabolic disease.
| Target | Fibroblast growth factor 19 (gene FGF19; UniProt O95750, human) |
|---|---|
| Clone | 24F22 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.0 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human SH-SY5Y cells, human U-87MG cells, human Caco-2 cells, human HepG2 cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FGF19.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes fibroblast growth factor 19 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in fetal brain, cartilage, retina, and adult gall bladder. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
