| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fibroblast growth factor 23;FGF-23;Fgf23; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived mouse FGF23 recombinant protein (Position: R48-V251). Mouse FGF23 shares 71.1% and 94.6% amino acid (aa) sequence identity with human and rat FGF23, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of Fgf23 (Fibroblast growth factor 23) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FGF23 Antibody Picoband® catalog # PB9868. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived mouse FGF23 recombinant protein (Position: R48-V251). Mouse FGF23 shares 71.1% and 94.6% amino acid (aa) sequence identity with human and rat FGF23, respectively. (reported region: R48-V251).
- Molecular weight context: reported MW: 34 kDa; calculated MW: 27758 MW
- Reactivity: Mouse
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Fibroblast growth factor 23; Fibroblast growth factor 23. Fibroblast growth factor 23?or?FGF23?is a?protein?that in humans is encoded by the?FGF23?gene. This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). Functional note: Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Acts ly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Upregulates EGR1 expression in the presence of KL. . Reported localization: Secreted . Secretion is dependent on O-glycosylation. . Expression/tissue context: Mainly expressed in the brain and thymus at low levels. In brain; preferentially expressed in the ventrolateral thalamic nucleus.
Research relevance and current trends
- Cancer: Researchers commonly examine how Fgf23 (Fibroblast growth factor 23) relates to this theme using model systems and orthogonal readouts.
- Cytoskeleton/ECM: Researchers commonly examine how Fgf23 (Fibroblast growth factor 23) relates to this theme using model systems and orthogonal readouts.
- Developmental Biology: Researchers commonly examine how Fgf23 (Fibroblast growth factor 23) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative Fgf23 (Fibroblast growth factor 23) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
