| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibroblast growth factor receptor 1;FGFR-1;2.7.10.1;Basic fibroblast growth factor receptor 1;BFGFR;bFGF-R-1;Fms-like tyrosine kinase 2;FLT-2;N-sam;Proto-oncogene c-Fgr;CD331;FGFR1;BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FGFR1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EHC-6 is a rabbit monoclonal antibody that recognizes fibroblast growth factor receptor 1 (gene FGFR1) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 91.9 kDa; the supplier reports observed bands at 120, 140 kDa.
Fibroblast growth factor receptor 1 (FGFR1) is a receptor tyrosine kinase that binds FGFs and activates MAPK, PI3K–AKT and PLCγ signaling. It controls cell proliferation, differentiation and migration and is essential for mesoderm formation, skeletal development and GnRH neuron development. FGFR1 amplifications and fusions occur in several cancers.
| Target | Fibroblast growth factor receptor 1 (gene FGFR1; UniProt P11362, human) |
|---|---|
| Clone | EHC-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 91.9 kDa |
| Observed MW | 120, 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FGFR1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places fibroblast growth factor receptor 1 in the cell membrane, nucleus, cytoplasm and cytoplasmic vesicle. Tissue expression noted by UniProt (human): Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
