| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Toll-like receptor 3; CD283; TLR3 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human FGFR3. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FGFR3 Antibody Picoband® is an antibody for FGFR3 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FGFR3 (toll-like receptor 3); UniProt: P22607
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 100 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FGFR3 Antibody Picoband® catalog # A00200-3.
Biological background
Biological context: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.
Expression and localization notes: cellular localization: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane. Early endosome., tissue context: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons..
Common research applications
- Western blotting (WB): Compare FGFR3 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FGFR3 in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify FGFR3-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Fibroblast growth factor receptor 3, also known as CD333, is a protein that in humans is encoded by the FGFR3 gene. This gene encodes a member of the fibroblast growth factor receptor(FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. The FGFR3 gene is mapped to the HD region on chromosome 4p16.3. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane. Early endosome.
- Tissue details: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
- Research category: Antiviral Signaling,Chemokines,Host-Virus Interaction,Immune System Diseases,Immunoglobulins,Immunology,Innate Immunity,Interspecies Interaction,Macrophage/Inflammation,Microbiology,NFKB Pathway,Nuclear Signaling,Receptors,Signal Transduction,Signaling Pathway,TLR Signaling
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
