| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Collagen alpha-1 (III) chain; COL3A1 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FGG, different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FGG Antibody Picoband® (monoclonal, 5H9) is an antibody for FGG detection raised in Mouse (Monoclonal, clone Clone: 5H9, Mouse IgG2b), with reported reactivity: Human. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FGG (collagen, type III, alpha 1); UniProt: P02679
- Antibody format: Mouse, Monoclonal, clone Clone: 5H9, Mouse IgG2b
- Molecular weight: 52 kDa
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FGG Antibody Picoband® (monoclonal, 5H9) catalog # M00790-3.
Biological background
Biological context: Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
Expression and localization notes: cellular localization: Extracellular matrix., tissue context: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells..
Common research applications
- Western blotting (WB): Compare FGG levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FGG in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify FGG-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Fibrinogen gamma chain, also known as FGG, is a human gene found on Chromosome 4. The protein encoded by this gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in transcript variants encoding different isoforms.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Extracellular matrix.
- Tissue details: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
- Research category: Collagen,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Mesenchymal Stem Cells,Signal Transduction,Stem Cells
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
