| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fibrinogen gamma chain; FGG |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FGG, different from the related mouse sequence by two amino acids, and from the related rat sequence by five amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 5H9 is a mouse monoclonal antibody that recognizes fibrinogen gamma chain (gene FGG) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 51.5 kDa, and the supplier reports an observed band at about 52 kDa.
Fibrinogen gamma chain (FGG) is one of the three chains of fibrinogen, the plasma protein that thrombin converts into fibrin. The gamma chain contains sites for fibrin cross-linking by factor XIII and for binding the platelet integrin αIIbβ3, so it is important for clot stability. It is studied in hemostasis, thrombosis and inflammation.
| Target | Fibrinogen gamma chain (gene FGG; UniProt P02679, human) |
|---|---|
| Clone | 5H9 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 51.5 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (ICC/IF): HEP3B cells.
Immunogen
Synthetic peptide from the N-terminal region of human FGG. It differs from the mouse sequence at two residues and from the rat sequence at five residues.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes fibrinogen gamma chain as a secreted protein. Tissue expression noted by UniProt (human): Detected in blood plasma (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
