| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Fibronectin;FN;Anastellin;Fn1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Fibronectin from human plasma. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Fibronectin Fn1 Monoclonal Antibody is an antibody targeting FN1. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: FN-15; isotype: Mouse IgG1; reactivity: Chicken,Human,Mouse; observed MW: 42 kDa; calculated MW: 272511 MW.
Boster Bio Anti-Fibronectin Fn1 Monoclonal Antibody catalog # MA1116. Tested in IHC, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse.
Key elements and design rationale
- Target: FN1 — Fibronectin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: FN-15; Isotype: Mouse IgG1
- Species reactivity: Chicken,Human,Mouse
- Molecular weight guidance: Observed: 42 kDa; Calculated: 272511 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). .
Scientific background (datasheet): Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.
Cellular localization (datasheet): Secreted, extracellular space, extracellular matrix.
Tissue details (datasheet): Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Sequence similarities (datasheet): Contains 12 fibronectin type-I domains.
Research relevance and current trends
- Commonly studied in contexts related to Actin,Actin, etc.,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
