Anti-Filamin A/FLNA Antibody Picoband® (monoclonal, 3F8)

Overview

Anti-Filamin A/FLNA Antibody Picoband® (monoclonal, 3F8) is an antibody for FLNA detection raised in Mouse (Monoclonal, clone Clone: 3F8, Mouse IgG1), with reported reactivity: Human. Commonly used in WB, IHC, IF, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: FLNA (CD5 molecule); UniProt: P21333; NCBI Gene: 2316
• Antibody format: Mouse, Monoclonal, clone Clone: 3F8, Mouse IgG1
• Molecular weight: 281 kDa
• Applications: WB, IHC, IF, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-Filamin A/FLNA Antibody Picoband® (monoclonal, 3F8) catalog # M00502-2.

Biological background

Biological context: May act as a receptor in regulating T-cell proliferation.

Expression and localization notes: cellular localization: Cell membrane; Single-pass type I membrane protein, tissue context: Brain, liver, placenta, lymphocytes and erythrocytes..

Common research applications

• Western blotting (WB): Compare FLNA levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of FLNA in tissue sections, considering fixation and antigen retrieval effects.
• Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
• Flow cytometry: Quantify FLNA-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: Filamin A, alpha (FLNA) is a protein that in humans is encoded by the FLNA gene. It is mapped to Xq28. The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Cell membrane; Single-pass type I membrane protein
• Tissue details: Brain, liver, placenta, lymphocytes and erythrocytes.
• Research category: Cell Adhesion,Cell Type Markers,Cytoskeleton/ECM,Hematopoietic Progenitors,Immunology,Integrins,Myeloid,Signal Transduction,Stem Cells

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.