| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Filensin;Beaded filament structural protein 1;Lens fiber cell beaded-filament structural protein CP 94;CP94;Bfsp1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Human and bovine lens filament enriched fraction (plasma membrane-cytoskeleton complex). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Filensin Bfsp1 Antibody (Monoclonal, FIL-7B10) is an antibody targeting BFSP1. Common applications include WB, IHC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: FIL-7B10; isotype: Mouse IgG1; reactivity: Bovine,Human; observed MW: 426 kDa; calculated MW: 68038 MW.
Boster Bio Anti-Filensin Bfsp1 Antibody (Monoclonal, FIL-7B10) catalog # MA1041. Tested in IHC, WB applications. This antibody reacts with Bovine, Human.
Key elements and design rationale
- Target: BFSP1 — Filensin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: FIL-7B10; Isotype: Mouse IgG1
- Species reactivity: Bovine,Human
- Molecular weight guidance: Observed: 426 kDa; Calculated: 68038 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol-1,4,5- trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA- mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC (By similarity). .
Scientific background (datasheet): Filensin, also known as beaded filament structure protein 1, have two major component BFSP1 and BESP2. Filensin gene is mapped at 20p12.1-p11.23. The sequence of the predicted 665-amino acid human protein is 62% and 50% identical to those of bovine and chicken filensin, respectively. However, it has less than 26% identity to other members of the intermediate filament (IF) family.
Cellular localization (datasheet): Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Detected adjacent to the cell membrane. .
Tissue details (datasheet): Detected in eye lens. .
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Neuroscience,Sensory System,Visual System.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
