| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Luciferin 4-monooxygenase;Luciferase;1.13.12.7; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from Firefly Luciferase |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-No Gene Name antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone DAB-6; isotype Rabbit IgG; reactivity: Firefly. Reported application contexts include WB, ICC, IF (as provided in the source record). Boster Bio Anti-Firefly Luciferase Rabbit Monoclonal Antibody catalog # MT0021. Tested in WB, ICC/IF applications. This antibody reacts with Firefly.
Key elements and design rationale
- Target: No Gene Name (Luciferin 4-monooxygenase).
- Antibody format: Monoclonal; clone DAB-6; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Firefly (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
No Gene Name is a commonly studied target in molecular and cellular biology. Functional context (as provided): Produces green light with a wavelength of 562 nm. Reported cellular localization context: Peroxisome . Tissue expression notes (as provided): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Research relevance and current trends
- Research context keywords from the source record include: 2339,Epigenetics and Nuclear Signaling,Histones,Unmodified.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate No Gene Name antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect No Gene Name expression by Western blot in cell or tissue lysates, Localize No Gene Name by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 15 kDa; calculated MW: 60745 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 15 kDa
- Cellular localization (provided): Peroxisome .
- Tissue details (provided): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
