| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peptidyl-prolyl cis-trans isomerase FKBP10; PPIase FKBP10; 65 kDa FK506-binding protein; 65 kDa FKBP; FKBP-65; FK506-binding protein 10; FKBP-10; Immunophilin FKBP65; Rotamase; FKBP10; FKBP65 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FKBP10 recombinant protein (Position: R29-E532). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes peptidyl-prolyl cis-trans isomerase FKBP10 in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 64.2 kDa, and the supplier reports an observed band at about 72 kDa.
FKBP10 (FKBP65) is an endoplasmic reticulum peptidyl-prolyl cis-trans isomerase that acts as a chaperone for collagen folding and processing. Mutations in FKBP10 cause a recessive form of osteogenesis imperfecta and Bruck syndrome, so it is studied in collagen biosynthesis, bone disease and fibrosis.
| Target | Peptidyl-prolyl cis-trans isomerase FKBP10 (gene FKBP10; UniProt Q96AY3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 64.2 kDa |
| Observed MW | 72 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A375 cells, human 293T cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Samples with a confirmed signal (flow cytometry): A549 cells.
Immunogen
Recombinant human FKBP10 fragment (Arg29–Glu532), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places peptidyl-prolyl cis-trans isomerase FKBP10 in the endoplasmic reticulum lumen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhang Lu et al. (2025) Therapeutic effect of pH responsive Magainin II modified azithromycin plus curcumin micelles in different depth models of MRSA infection. Scientific Reports. 10.1038/s41598-025-92384-z
