| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Peptidyl-prolyl cis-trans isomerase FKBP1A;PPIase FKBP1A;5.2.1.8;12 kDa FK506-binding protein;12 kDa FKBP;FKBP-12;Calstabin-1;FK506-binding protein 1A;FKBP-1A;Immunophilin FKBP12;Rotamase;FKBP1A;FKBP1, FKBP12; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human FKBP12 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-FKBP1A antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AAFH-6; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF (as provided in the source record). Boster Bio Anti-FKBP12 Rabbit Monoclonal Antibody catalog # M04492. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: FKBP1A (Peptidyl-prolyl cis-trans isomerase FKBP1A).
- Antibody format: Monoclonal; clone AAFH-6; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
FKBP1A (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Keeps in an inactive conformation TGFBR1, the TGF-beta type I serine/threonine kinase receptor, preventing TGF-beta receptor activation in absence of ligand. Recruites SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. May modulate the RYR1 calcium channel activity. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. . Reported cellular localization context: Cytoplasm. Tissue expression notes (as provided): Specifically expressed in stromal cells of breast carcinomas.
Research relevance and current trends
- Research context keywords from the source record include: Immunology.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
Workflow ideas (metafield): Validate FKBP1A antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect FKBP1A expression by Western blot in cell or tissue lysates, Localize FKBP1A by immunofluorescence/immunocytochemistry in cultured cells
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 12 kDa; calculated MW: 11951 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 12 kDa
- Cellular localization (provided): Cytoplasm.
- Tissue details (provided): Specifically expressed in stromal cells of breast carcinomas.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
