| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peptidyl-prolyl cis-trans isomerase FKBP5; PPIase FKBP5; 51 kDa FK506-binding protein; 51 kDa FKBP; FKBP-51; 54 kDa progesterone receptor-associated immunophilin; Androgen-regulated protein 6; FF1 antigen; FK506-binding protein 5; FKBP-5; FKBP54; p54; HSP90-binding immunophilin; Rotamase; FKBP5; AIG6; FKBP51 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FKBP51 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFBE-6 is a rabbit monoclonal antibody that recognizes peptidyl-prolyl cis-trans isomerase FKBP5 in human and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 51.2 kDa, and the supplier reports an observed band at about 51 kDa.
FKBP51 (FKBP5) is an HSP90 co-chaperone and peptidyl-prolyl isomerase that binds steroid hormone receptor complexes. It lowers glucocorticoid receptor sensitivity and is itself strongly induced by glucocorticoids, forming a negative feedback loop. FKBP5 is widely studied in stress hormone signaling and stress-related psychiatric research.
| Target | Peptidyl-prolyl cis-trans isomerase FKBP5 (gene FKBP5; UniProt Q13451, human) |
|---|---|
| Clone | AFBE-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.2 kDa |
| Observed MW | 51 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human Caco-2 cells, human HeLa cells, human Jurkat cells, human HepG2 cells, rat thymus tissue, rat PC-12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colorectal adenocarcinoma tissue, human liver cancer tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human FKBP51.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places peptidyl-prolyl cis-trans isomerase FKBP5 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Widely expressed, enriched in testis compared to other tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
