| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein; ALOX5AP |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FLAP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24A38 is a rabbit monoclonal antibody that recognizes arachidonate 5-lipoxygenase-activating protein (gene ALOX5AP) in human samples and is validated for Western blot. The predicted molecular weight is 18.2 kDa, and the supplier reports an observed band at about 16 kDa.
FLAP (ALOX5AP, 5-lipoxygenase-activating protein) is a nuclear membrane protein that binds arachidonic acid and presents it to 5-lipoxygenase. It is required for cellular leukotriene production and is the target of FLAP inhibitors such as MK-886 used in inflammation research.
| Target | Arachidonate 5-lipoxygenase-activating protein (gene ALOX5AP; UniProt P20292, human) |
|---|---|
| Clone | 24A38 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 18.2 kDa |
| Observed MW | 16 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human FLAP.
Reactivity Notes
The supplier lists reactivity with human. UniProt places arachidonate 5-lipoxygenase-activating protein in the nuclear membrane and endoplasmic reticulum membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
