| Field | Specification |
|---|---|
| Target | |
| Alternative names | Fms-related tyrosine kinase 3 ligand; Flt3 ligand; Flt3L; SL cytokine; Flt3lg; Flt3l; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Flt3 ligand recombinant protein (Position: T28-Q189). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes fms-related tyrosine kinase 3 ligand (gene Flt3lg) in mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 26.1 kDa, and the supplier reports an observed band at about 26 kDa.
FLT3 ligand (FLT3LG, Flt3L) is a hematopoietic cytokine that activates the FLT3 receptor on early progenitor cells. It works together with other growth factors to expand hematopoietic progenitors and is essential for dendritic cell development, while also supporting B-cell development. Flt3L is widely used to generate dendritic cells in culture.
| Target | Fms-related tyrosine kinase 3 ligand (gene Flt3lg; UniProt P49772, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 26.1 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse Flt3 ligand fragment (Thr28–Gln189), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places fms-related tyrosine kinase 3 ligand in the cell membrane and SUBCELLULAR LOCATION:. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
