| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Formin-1;Limb deformity protein homolog;FMN1;FMN, LD; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human FMN1 recombinant protein (Position: N1195-N1419). Human FMN1 shares 93.8% amino acid (aa) sequence identity with mouse FMN1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FMN1 (BAG family molecular chaperone regulator 5) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FMN1 Antibody Picoband® catalog # A07520. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human FMN1 recombinant protein (Position: N1195-N1419). Human FMN1 shares 93.8% amino acid (aa) sequence identity with mouse FMN1. (reported region: N1195-N1419).
- Molecular weight context: reported MW: 158 kDa; calculated MW: 157578 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
BAG family molecular chaperone regulator 5; Formin-1. Formins, such as FMN1, are actin-nucleating proteins involved in cell polarity, cytokinesis, cell migration, and transcriptional activity. This FMN1 gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene. Functional note: Plays a role in the formation of adherens junction and the polymerization of linear actin cables. . Reported localization: Nucleus . Cytoplasm . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Localization to the adherens junctions is alpha-catenin-dependent. Also localizes to F-actin bundles originating from adherens junctions and to microtubules (By similarity). . Expression/tissue context: Expressed ubiquitously.
Research relevance and current trends
- Actin Assembly: Researchers commonly examine how FMN1 (BAG family molecular chaperone regulator 5) relates to this theme using model systems and orthogonal readouts.
- Actin: Researchers commonly examine how FMN1 (BAG family molecular chaperone regulator 5) relates to this theme using model systems and orthogonal readouts.
- etc.: Researchers commonly examine how FMN1 (BAG family molecular chaperone regulator 5) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FMN1 (BAG family molecular chaperone regulator 5) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of FMN1 (BAG family molecular chaperone regulator 5) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
