| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dimethylaniline monooxygenase [N-oxide-forming] 2;1.14.13.8;Dimethylaniline oxidase 2;FMO 1B1;Pulmonary flavin-containing monooxygenase 2;FMO 2;FMO2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FMO2, different from the related mouse and rat sequences by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FMO2 (Dimethylaniline monooxygenase [N-oxide-forming] 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FMO2 Antibody Picoband® catalog # PB9760. Tested in WB applications. This antibody reacts with Human;Mouse;Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human FMO2, different from the related mouse and rat sequences by two amino acids.
- Molecular weight context: reported MW: 61 kDa; calculated MW: 61 kDa
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Dimethylaniline monooxygenase [N-oxide-forming] 2; Dimethylaniline monooxygenase [N-oxide-forming] 2. Dimethylaniline monooxygenase [N-oxide-forming] 2 is an enzyme that in humans is encoded by the FMO2 gene. This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants. Functional note: Catalyzes the N-oxidation of certain primary alkylamines to their oximes via an N-hydroxylamine intermediate. Inactive toward certain tertiary amines, such as imipramine or chloropromazine. Can catalyze the S-oxidation of methimazole. The truncated form is catalytically inactive. . Reported localization: Microsome membrane. Endoplasmic reticulum membrane. Expression/tissue context: Expressed in lung (at protein level). Expressed predominantly in lung, and at a much lesser extent in kidney. Also expressed in fetal lung, but not in liver, kidney and brain. .
Research relevance and current trends
- Cancer: Researchers commonly examine how FMO2 (Dimethylaniline monooxygenase [N-oxide-forming] 2) relates to this theme using model systems and orthogonal readouts.
- Drug Metabolism: Researchers commonly examine how FMO2 (Dimethylaniline monooxygenase [N-oxide-forming] 2) relates to this theme using model systems and orthogonal readouts.
- Metabolic Signaling Pathways: Researchers commonly examine how FMO2 (Dimethylaniline monooxygenase [N-oxide-forming] 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FMO2 (Dimethylaniline monooxygenase [N-oxide-forming] 2) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
