| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dimethylaniline monooxygenase [N-oxide-forming] 5;1.14.13.8;Dimethylaniline oxidase 5;Hepatic flavin-containing monooxygenase 5;FMO 5;FMO5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FMO5, different from the related rat sequence by one amino acid, and different from the related mouse sequence by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FMO5 Antibody Picoband® is an antibody targeting FMO5. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Human; observed MW: 60 kDa; calculated MW: 60221 MW.
Boster Bio Anti-FMO5 Antibody catalog # PA2108. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: FMO5 — Dimethylaniline monooxygenase [N-oxide-forming] 5
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 60 kDa; Calculated: 60221 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme.
Scientific background (datasheet): Dimethylaniline monooxygenase[N-oxide-forming] 5 also known as FMO 5 is an enzyme that in humans is encoded by the FMO5 gene. By fluorescence in situ hybridization, this gene was assigned to human chromosome 1q21.1. FMO5 is not an efficient drug-metabolizing enzyme and that it may have an alternative physiologic role. FMO5 transcripts play an important role in progesterone-regulated in breast cancer cells specifically under the control of the progesterone receptor B-isoform.
Cellular localization (datasheet): Microsome membrane. Endoplasmic reticulum membrane.
Tissue details (datasheet): Expressed in fetal and adult liver.
Sequence similarities (datasheet): Belongs to the FMO family.
Research relevance and current trends
- Commonly studied in contexts related to Drug Metabolism,Metabolic Signaling Pathways,Metabolism,Pathways and Processes,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
