| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein fosB;G0/G1 switch regulatory protein 3;FOSB;G0S3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Fos B, different from the related mouse sequence by two amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FOSB (Protein fosB) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Fos B/FOSB Antibody catalog # RP1086. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human Fos B, different from the related mouse sequence by two amino acids.
- Molecular weight context: reported MW: 35 kDa; calculated MW: 35928 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein fosB; Protein fosB. FOSB, FBJ murine osteosarcoma viral oncogene homolog B, is a protein that, in humans, is encoded by the FOSB gene. FOSB is a member of Fos gene family which consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. The FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. The FOSB gene is mapped to 19q13.32. Delta FOSB is a truncated splice variant of FOSB. Delta FosB has been implicated in the development of drug addiction and control of the reward system in the brain, and is linked to changes in a number of other gene products such as CREB and sirtuins. Delta FosB also regulates the commitment of mesenchymal precursor cells to the adipocyte or osteoblast lineage. Functional note: FosB interacts with Jun proteins enhancing their DNA binding activity. Reported localization: Nucleus. Expression/tissue context: Testis-specific. Expressed in prostate cancer (at protein level). .
Research relevance and current trends
- Cancer: Researchers commonly examine how FOSB (Protein fosB) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how FOSB (Protein fosB) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how FOSB (Protein fosB) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FOSB (Protein fosB) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of FOSB (Protein fosB) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Belongs to the bZIP family. Fos subfamily.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
