| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte nuclear factor 3-alpha;HNF-3-alpha;HNF-3A;Forkhead box protein A1;Transcription factor 3A;TCF-3A;FOXA1;HNF3A, TCF3A; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FOXA1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOEE-6 is a rabbit monoclonal antibody that recognizes hepatocyte nuclear factor 3-alpha (gene FOXA1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 49.1 kDa, and the supplier reports an observed band at about 49 kDa.
FOXA1 (HNF-3α) is a forkhead transcription factor that acts as a pioneer factor: it can bind compacted chromatin and open it so other transcription factors can reach their targets. It is important in development of the liver, pancreas, lung and prostate and guides estrogen and androgen receptor binding in breast and prostate cancer.
| Target | Hepatocyte nuclear factor 3-alpha (gene FOXA1; UniProt P55317, human) |
|---|---|
| Clone | AOEE-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 49.1 kDa |
| Observed MW | 49 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human MCF-7 cells, human RT4 cells, human Caco-2 cells, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human esophageal squamous cells carcinoma tissue, human lung cancer tissue, mouse liver tissue, rat liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human FOXA1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hepatocyte nuclear factor 3-alpha in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in prostate and ESR1-positive breast tumors. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yutong Yan et al. (2022) Estradiol Regulates the Expression and Secretion of Antimicrobial Peptide S100A7 via the ERK1/2-Signaling Pathway in Goat Mammary Epithelial Cells. Animals. 10.3390/ani12223077
Haihong Li et al. (2018) Expression and localization of Forkhead transcription factor A1 in the three-dimensional reconstructed eccrine sweat glands. ACTA Histochemica. 10.1016/j.acthis.2018.06.003
