| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte nuclear factor 3-beta;HNF-3-beta;HNF-3B;Forkhead box protein A2;Transcription factor 3B;TCF-3B;FOXA2;HNF3B, TCF3B; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human FOXA2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAAH-6 is a rabbit monoclonal antibody that recognizes hepatocyte nuclear factor 3-beta (gene FOXA2) in human and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 48.3 kDa, and the supplier reports an observed band at about 50 kDa.
FOXA2 (HNF-3β) is a forkhead transcription factor and pioneer factor that opens chromatin for other regulators. It is essential for forming the node, notochord and endoderm in early development and for liver, pancreas and lung development, and it regulates metabolic genes in the adult liver. FOXA2 is a common endoderm marker in stem cell research.
| Target | Hepatocyte nuclear factor 3-beta (gene FOXA2; UniProt Q9Y261, human) |
|---|---|
| Clone | AAAH-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 48.3 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HepG2 cells, human PC-3 cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human FOXA2.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places hepatocyte nuclear factor 3-beta in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
