| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein G1; Brain factor 1; BF-1; BF1; Brain factor 2; BF-2; BF2; hBF-2; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; Forkhead-related protein FKHL1; HFK1; Forkhead-related protein FKHL2; HFK2; Forkhead-related protein FKHL3; HFK3; FOXG1; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FOXG1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes forkhead box protein G1 (gene FOXG1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 52.4 kDa, and the supplier reports an observed band at about 52 kDa.
FOXG1 is a forkhead transcriptional repressor that is essential for development of the telencephalon, the part of the brain that forms the cerebral cortex. It controls the proliferation and differentiation of cortical progenitor cells and helps divide the forebrain into regions. FOXG1 mutations cause FOXG1 syndrome, a severe neurodevelopmental disorder.
| Target | Forkhead box protein G1 (gene FOXG1; UniProt P55316, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.4 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human U251 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human FOXG1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places forkhead box protein G1 in the nucleus. Tissue expression noted by UniProt (human): Expression is restricted to the neurons of the developing telencephalon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
