| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein L2; FOXL2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human FOXL2, identical to the related mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects forkhead box protein L2 (gene FOXL2) in human, mouse and rat samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 38.8 kDa, and the supplier reports an observed band at about 50 kDa.
FOXL2 is a forkhead transcription factor essential for ovarian development and for maintaining ovarian granulosa cell identity; it represses the testis-promoting gene SOX9. FOXL2 mutations cause blepharophimosis–ptosis–epicanthus inversus syndrome, and a recurrent somatic mutation is characteristic of adult granulosa cell tumors.
| Target | Forkhead box protein L2 (gene FOXL2; UniProt P58012, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.8 kDa |
| Observed MW | 50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human Jurkat cells, rat ovary tissue, mouse ovary tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, mouse ovary tissue, rat ovary tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): THP-1 cells.
Immunogen
Synthetic peptide from the C-terminal region of human FOXL2. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places forkhead box protein L2 in the nucleus. Tissue expression noted by UniProt (human): In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuanyuan Hou et al. (2023) MFN2 regulates progesterone biosynthesis and proliferation of granulosa cells during follicle selection in hens. Journal of Cellular Physiology. 10.1002/jcp.31143
Xuzhao Wang et al. (2025) Chromatin deactivation in pre-granulosa cells contributes to primordial follicle formation. Journal of Biological Chemistry. 10.1016/j.jbc.2025.110598
Xiaotong Guo et al. (2024) BMP2 is required for granulosa cell proliferation and primordial follicle activation in chicken. Poultry Science. 10.1016/j.psj.2024.104716
Sahin Hakan et al. (2025) Oxytocin receptor expression in developing mouse gonads and its modulation by high-dose folic acid. Journal of Molecular Histology. 10.1007/s10735-025-10621-1
Yueqiu Luo et al. (2015) microRNA133a Targets Foxl2 and Promotes Differentiation of C2C12 into Myogenic Progenitor Cells. DNA and Cell Biology. 10.1089/dna.2014.2522
