| Field | Specification |
|---|---|
| Target | |
| Alternative names | Forkhead box protein M1;Forkhead-related protein FKHL16;Hepatocyte nuclear factor 3 forkhead homolog 11;HFH-11;HNF-3/fork-head homolog 11;M-phase phosphoprotein 2;MPM-2 reactive phosphoprotein 2;Transcription factor Trident;Winged-helix factor from INS-1 cells;FOXM1;FKHL16, HFH11, MPP2, WIN; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human FOXM1 recombinant protein (Position: A411-Q763). Human FOXM1 shares 75% and 76% amino acid (aa) sequences identity with mouse and rat FOXM1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects forkhead box protein M1 (gene FOXM1) in human samples and is validated for Western blot. The predicted molecular weight is 84.3 kDa, and the supplier reports an observed band at about 36 kDa.
FOXM1 is a forkhead transcription factor that activates genes required for DNA replication and mitosis, such as cyclin B1 and PLK1. It is expressed in proliferating cells, supports DNA damage responses, and is overexpressed in many cancers, so it is studied as a driver of cell proliferation and tumor growth.
| Target | Forkhead box protein M1 (gene FOXM1; UniProt Q08050, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 84.3 kDa |
| Observed MW | 36 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human COLO320 cells, human SW620 cells, human SKOV cells, human MCD-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human FOXM1 fragment (Ala411–Gln763), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 75% and 76%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Expressed in thymus, testis, small intestine, colon followed by ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
