| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Forkhead box protein M1;Forkhead-related protein FKHL16;Hepatocyte nuclear factor 3 forkhead homolog 11;HFH-11;HNF-3/fork-head homolog 11;M-phase phosphoprotein 2;MPM-2 reactive phosphoprotein 2;Transcription factor Trident;Winged-helix factor from INS-1 cells;FOXM1;FKHL16, HFH11, MPP2, WIN; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human FOXM1 recombinant protein (Position: A411-Q763). Human FOXM1 shares 75% and 76% amino acid (aa) sequences identity with mouse and rat FOXM1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of FOXM1 (Forkhead box protein M1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-FOXM1 Antibody Picoband® catalog # PB9194. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human FOXM1 recombinant protein (Position: A411-Q763). Human FOXM1 shares 75% and 76% amino acid (aa) sequences identity with mouse and rat FOXM1, respectively. (reported region: A411-Q763).
- Molecular weight context: reported MW: 36 kDa; calculated MW: 84283 MW
- Reactivity: Human
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Forkhead box protein M1; Forkhead box protein M1. Forkhead box protein M1, also called FOXM1, is a protein that in humans is encoded by the FOXM1 gene. It is mapped to 12p13.33. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases and also in the control of cell proliferation. FOXM1 gene is now known as a human proto-oncogene. Abnormal upregulation of FOXM1 is involved in the oncogenesis of basal cell carcinoma, the most common human cancer worldwide. It was hypothesized that FOXM1 induces cancer initiation through stem/progenitor cell expansion. What's more, FOXM1 has been shown to modulate the epigenome. It was found that overexpression of FOXM1 brain washes normal cells to adopt cancer-like epigenome. Functional note: Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response. . Reported localization: Nucleus. Expression/tissue context: Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.
Research relevance and current trends
- Cancer: Researchers commonly examine how FOXM1 (Forkhead box protein M1) relates to this theme using model systems and orthogonal readouts.
- Domain Families: Researchers commonly examine how FOXM1 (Forkhead box protein M1) relates to this theme using model systems and orthogonal readouts.
- Epigenetics and Nuclear Signaling: Researchers commonly examine how FOXM1 (Forkhead box protein M1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative FOXM1 (Forkhead box protein M1) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Family / similarity context: Contains 1 fork-head DNA-binding domain.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
