| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human FOXN1, which shares 85.7% amino acid (aa) sequence identity with mouse FOXN1. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-FOXN1 Antibody Picoband® is an antibody for FOXN1 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: FOXN1 (group specific component); UniProt: O15353
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 69 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-FOXN1 Antibody Picoband® catalog # A03369-2.
Biological background
Biological context: Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Expression and localization notes: cellular localization: Secreted..
Common research applications
- Western blotting (WB): Compare FOXN1 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of FOXN1 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify FOXN1-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: The FOXN1 gene encodes a transcription factor that regulates the development of epithelial cells in the thymus and skin. FOXN1 is expressed in thymic epithelial cell precursors which interact with T-cell progenitors for T-cell development in the thymus, and thus plays an essential role in thymic development beginning in the embryo. FOXN1 is also expressed in keratinocytes, hair follicles, epithelial cells in the nail matrix bed, and cells of the choroid plexus.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Research category: Actin Binding Proteins,Actin, etc.,Cancer,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction,Tumor Antigens,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
